Tuning genetic control through promoter engineering

Tuning genetic control through promoter engineering.

Gene function is typically evaluated by sampling the continuum of gene expression at only a few discrete points corresponding to gene knockout or overexpression. We argue that this characterization is incomplete and present a library of engineered promoters of varying strengths obtained through mutagenesis of a constitutive promoter. A multifaceted characterization of the library, especially at...

Fine Tuning of the Lactate and Diacetyl Production through Promoter Engineering in Lactococcus lactis

Lactococcus lactis is a well-studied bacterium widely used in dairy fermentation and capable of producing metabolites with organoleptic and nutritional characteristics. For fine tuning of the distribution of glycolytic flux at the pyruvate branch from lactate to diacetyl and balancing the production of the two metabolites under aerobic conditions, a constitutive promoter library was constructed...

Genetic Algorithms in Control Systems Engineering

Genetic algorithms (GAs) are global, parallel, stochastic search methods, founded on Darwinian evolutionary principles. Many variations exist, including genetic programming and multiobjective algorithms. During the last decade GAs have been applied in a variety of areas, with varying degrees of success within each. A significant contribution has been made within control systems engineering. GAs...

Genetic Algorithm Tools for Control Systems Engineering

Engineering infection control through facility design.

Many medical centers have modified their facility design to provide a safer environment for patients. From an infection control perspective, the primary objective of hospital design is to place the patient at no risk for infection while hospitalized. We describe historical landmarks about hospital design, modern facility design, and specific designs to prevent acquisition and spread of infectio...